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superscriptiii first-strand synthesis supermix  (Thermo Fisher)


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    Thermo Fisher superscriptiii first-strand synthesis supermix
    Superscriptiii First Strand Synthesis Supermix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscriptiii+first-strand+synthesis+supermix/high+capacity+cdna+reverse+transcription+kit/pmc10930802-88-6-10
    Average 90 stars, based on 1 article reviews
    superscriptiii first-strand synthesis supermix - by Bioz Stars, 2026-09
    90/100 stars

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    Reverse Transcription:

    Article Title: Resolving Cell Cycle Speed in One Snapshot with a Live-Cell Fluorescent Reporter
    Article Snippet: .. Total mRNA was extracted with TRIzol® Reagent (Ambion) and reverse transcribed into cDNA using SuperScriptIII First-Strand Synthesis SuperMix (Invitrogen) according to the product manual. .. For quantitative real-time PCR, cDNA and gene-specific primers were mixed with iQ SYBR®Green Supermix (Bio-Rad) and carried out using a Bio-Rad CFX384 Real-Time PCR System.

    Article Title: Transcriptome profiling of regulatory T cells from children with transient hypogammaglobulinemia of infancy.
    Article Snippet: .. Shortly, reverse transcription was performed using SuperScriptIII First-Strand Synthesis SuperMix (Invitrogen, ThermoFisher Scientific, USA). .. PCR reactions were performed in duplicates using TaqMan Gene Expression Master Mix (ThermoFisher Scientific, USA) and appropriate assays (ThermoFisher Scientific, USA): Eukaryotic 18S rRNA (Entrez gene ID: HSRRN18S, assay ID: Hs03003631_g1), FOXP3 (Entrez gene ID:50943, assay ID: Hs01085834_m1), IL2RA (Entrez gene ID: 3559, assay ID: Hs00166229_m1), CCR7 (Entrez gene ID: 1236, assay ID: Hs01013469_m1), and LEF1 (Entrez gene ID: 51176, assay ID: Hs01547250_m1).

    Article Title: Gene Signature of Regulatory T Cells Isolated from Children with Selective IgA Deficiency and Common Variable Immunodeficiency
    Article Snippet: .. Briefly, reverse transcription was performed using SuperScriptIII First-Strand Synthesis SuperMix (Invitrogen, ThermoFisher Scientific, Waltham, MA, USA). .. PCR reactions were performed in duplicates using TaqMan Gene Expression Master Mix (ThermoFisher Scientific, MA, USA) and appropriate assays containing the following primers and probes (ThermoFisher Scientific, USA): Eukaryotic 18S rRNA (assay ID: Hs03003631_g1), FOXP3 (assay ID: Hs01085834_m1), LEF1 (assay ID: Hs01547250_m1), and MAPK3 (assay ID: Hs00385075_m1).

    Article Title: Clinical Features of Nivolumab-Induced Thyroiditis: A Case Series Study.
    Article Snippet: Th is p ap er h as b ee n pe er -re vi ew ed a nd a cc ep te d fo r p ub lic at io n, b ut h as y et to u nd er go c op ye di tin g an d pr oo f c or re ct io n. T he fi na l p ub lis he d ve rs io n m ay d iff er fr om th is pr oo f. Clinical Features of Nivolumab‐Induced Thyroiditis: A Case Series Study Author Ichiro Yamauchi, Yoriko Sakane, Yorihide Fukuda, Toshihito Fujii, Daisuke Taura, Masakazu Hirata, Keisho Hirota, Yohei Ueda, Yugo Kanai, Yui Yamashita, Eri Kondo, Masakatsu Sone, Akihiro Yasoda, Nobuya Inagaki Department of Diabetes, Endocrinology and Nutrition, Kyoto University Graduate School of Medicine, 54 Kawaharacho, Shogoin, Sakyo‐ku, Kyoto 606‐8507, Japan.. Preemptive Medicine and Lifestyle Disease Research Center, Kyoto University Hospital, 54 Kawaharacho, Shogoin, Sakyo‐ku, Kyoto 606‐8507, Japan Running Title Nivolumab‐Induced Thyroiditis

    Article Title: A deleterious Na v 1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients
    Article Snippet: Total RNA from samples of interest was purified using the RNeasy kit (Qiagen) and the RNase-free DNase Set (Qiagen). .. After normalization for concentration, RNA was reverse-transcribed into cDNA using the SuperScriptIII First-strand Synthesis SuperMix and random hexamer primers (Invitrogen). .. Real-time PCR for the majority of the cDNA samples was performed using the FastStart Universal SYBR Green Master (Rox) mix (Roche) on a Mastercycler realplex machine (Eppendorf) or a ViiA 7 machine (Applied Biosystems).

    Article Title: Clinical and Molecular Data Define a Diagnosis of Arrhythmogenic Cardiomyopathy in a Carrier of a Brugada-Syndrome-Associated PKP2 Mutation
    Article Snippet: Pictures were captured with an Axiovert microscope (Zeiss, Oberkochen, Germany) and quantified with AxioVision Rel.4.8. (Zeiss, Oberkochen, Germany). .. Total RNA was extracted using Trizol (ThermoFisher Scientific, Waltham, MS, USA) and reverse transcribed with SuperscriptIII First-Strand Synthesis SuperMix (Invitrogen, Carlsbad, CA, USA). .. Quantitative real time-polymerase chain reaction (qRT-PCR) was performed in duplicate using 10 ng of cDNA, the iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, California, United States) and the following primers: PLIN1-FW : 5′-CATTGAGAAGGTGGTGGAGTA-3′ PLIN1-REV : 5′-CTTGGCCTTGGGAGACTT-3′ PPARγ-FW : 5′-ACATAAAGTCCTTCCCGCTGACCA-3′ PPARγ-REV : 5′-AAACTGGCAGCCCTGAAAGATGC -3′ GAPDH- FW : 5′- ATGTTCGTCATGGGTGTGAA-3′ GAPDH- REV : 5′- GTCTTCTGGGTGGCAGTGAT-3′

    other:

    Article Title: The Dark Side of Microbial Processes: Accumulation of Nitrate During Storage of Surface Water in the Dark and the Underlying Mechanism
    Article Snippet: Synthesis of cDNA from the RNA samples was performed using the Invitrogen SuperscriptIII First-Strand Synthesis Supermix (ThermoFisher Scientific, Waltham, MA). qPCR was performed using the KAPA SYBR Fast Universal 2× qPCR Master Mix (Kapabiosystems) on a StepOne Plus real-time PCR system.

    Isolation:

    Article Title: Embryonic erythropoiesis and hemoglobin switching require transcriptional repressor ETO2 to modulate chromatin organization
    Article Snippet: RNA was isolated using the RNeasy mini kit (QIAGEN, 74104) for K562 cells, PicoPure RNA isolation kit (Thermo Fisher Scientific, KIT0202) for fetal liver or yolk sac cells, and Mouse RiboPure-Blood RNA Isolation Kit (Thermo Fisher Scientific, AM1951) for blood samples. .. After RNA isolation, DNaseI was used to remove genomic DNA following manufacturers’ protocols (Roche, 03539121103). cDNA was generated using the SuperscriptIII First-Strand Synthesis SuperMix (ThermoFisher Scientific, 18080-400) with random hexamers. .. Quantitative PCR was conducted using iTag Universal SYBR Green Super Mix (BioRad, 172-5120) and the 7900HT Real-Time PCR System (Applied Biosystems).

    Generated:

    Article Title: Embryonic erythropoiesis and hemoglobin switching require transcriptional repressor ETO2 to modulate chromatin organization
    Article Snippet: RNA was isolated using the RNeasy mini kit (QIAGEN, 74104) for K562 cells, PicoPure RNA isolation kit (Thermo Fisher Scientific, KIT0202) for fetal liver or yolk sac cells, and Mouse RiboPure-Blood RNA Isolation Kit (Thermo Fisher Scientific, AM1951) for blood samples. .. After RNA isolation, DNaseI was used to remove genomic DNA following manufacturers’ protocols (Roche, 03539121103). cDNA was generated using the SuperscriptIII First-Strand Synthesis SuperMix (ThermoFisher Scientific, 18080-400) with random hexamers. .. Quantitative PCR was conducted using iTag Universal SYBR Green Super Mix (BioRad, 172-5120) and the 7900HT Real-Time PCR System (Applied Biosystems).

    Concentration Assay:

    Article Title: A deleterious Na v 1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients
    Article Snippet: Total RNA from samples of interest was purified using the RNeasy kit (Qiagen) and the RNase-free DNase Set (Qiagen). .. After normalization for concentration, RNA was reverse-transcribed into cDNA using the SuperScriptIII First-strand Synthesis SuperMix and random hexamer primers (Invitrogen). .. Real-time PCR for the majority of the cDNA samples was performed using the FastStart Universal SYBR Green Master (Rox) mix (Roche) on a Mastercycler realplex machine (Eppendorf) or a ViiA 7 machine (Applied Biosystems).

    Random Hexamer:

    Article Title: A deleterious Na v 1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients
    Article Snippet: Total RNA from samples of interest was purified using the RNeasy kit (Qiagen) and the RNase-free DNase Set (Qiagen). .. After normalization for concentration, RNA was reverse-transcribed into cDNA using the SuperScriptIII First-strand Synthesis SuperMix and random hexamer primers (Invitrogen). .. Real-time PCR for the majority of the cDNA samples was performed using the FastStart Universal SYBR Green Master (Rox) mix (Roche) on a Mastercycler realplex machine (Eppendorf) or a ViiA 7 machine (Applied Biosystems).



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